other:
Plasmid Preparation:Article Title: The Bordetella effector protein BteA induces host cell death by disruption of calcium homeostasis
Article Snippet: .. Plasmid Description Reference pBBRI MCS lacPOZ’ mob+, broad-host cloning vector, CmR (11, 12) pBBRI-PgroESmNG pBBRI vector with BbRB50 promotor GroES (PgroES) and coding sequence of the mNeonGreen protein (mNG) This study pBBRI-PfhaBmSc pBBRI vector with BpTohamaI filamentous hemagglutinin promotor (pfhaB) and coding sequence of the mScarlet protein (mSc) (13) export&KDELmScarlet ER-mScarletI, pEGFP-N1-derived vector encoding mScarlet fluorescent protein targeted to mitochondria, Addgene item # 137805 (14) 4xmtsmNeonGreen 4xmts-mNeonGreen, pEGFP-N1-derived vector encoding mNeonGreen fluorescent protein targeted to mitochondria, Addgene item # 98876 (14) GeCKO v2 sublibrary A Human CRISPR Knockout Pooled Library (GeCKO v2) in lentiGuide-Puro A, Catalog #1000000049, Addgene item #52959 (15) GeCKO v2 sublibrary B Human CRISPR Knockout Pooled Library (GeCKO v2) in lentiGuide-Puro B, Catalog #1000000049, Addgene item #52960 (15) pCMV-VSV-G Vector encoding envelope protein for producing lentiviral and MuLV retroviral particles, Addgene item #8454 (16) psPAX2 2nd generation lentiviral packaging plasmid, Addgene item #12260 Addgene, Trono Lab unpublished ER-LAR-GECO CMV-ER-LAR-GECO1, low affinity red intensiometric genetically encoded Ca2+ indicator targeted to ER, Addgene item #61244 (17) mito-LARGECO CMV-mito-LAR-GECO1.2, low affinity red intensiometric genetically encoded Ca2+ indicator targeted to mitochondria, Addgene item #61245 (17) mito-mhYFP Mito-mhYFP, pcDNA3.1-derived vector encoding monomeric hyperfolder YFP fluorescent protein targeted to mitochondria, Addgene item #186526 (18) ..
Cloning:Article Title: The Bordetella effector protein BteA induces host cell death by disruption of calcium homeostasis
Article Snippet: .. Plasmid Description Reference pBBRI MCS lacPOZ’ mob+, broad-host cloning vector, CmR (11, 12) pBBRI-PgroESmNG pBBRI vector with BbRB50 promotor GroES (PgroES) and coding sequence of the mNeonGreen protein (mNG) This study pBBRI-PfhaBmSc pBBRI vector with BpTohamaI filamentous hemagglutinin promotor (pfhaB) and coding sequence of the mScarlet protein (mSc) (13) export&KDELmScarlet ER-mScarletI, pEGFP-N1-derived vector encoding mScarlet fluorescent protein targeted to mitochondria, Addgene item # 137805 (14) 4xmtsmNeonGreen 4xmts-mNeonGreen, pEGFP-N1-derived vector encoding mNeonGreen fluorescent protein targeted to mitochondria, Addgene item # 98876 (14) GeCKO v2 sublibrary A Human CRISPR Knockout Pooled Library (GeCKO v2) in lentiGuide-Puro A, Catalog #1000000049, Addgene item #52959 (15) GeCKO v2 sublibrary B Human CRISPR Knockout Pooled Library (GeCKO v2) in lentiGuide-Puro B, Catalog #1000000049, Addgene item #52960 (15) pCMV-VSV-G Vector encoding envelope protein for producing lentiviral and MuLV retroviral particles, Addgene item #8454 (16) psPAX2 2nd generation lentiviral packaging plasmid, Addgene item #12260 Addgene, Trono Lab unpublished ER-LAR-GECO CMV-ER-LAR-GECO1, low affinity red intensiometric genetically encoded Ca2+ indicator targeted to ER, Addgene item #61244 (17) mito-LARGECO CMV-mito-LAR-GECO1.2, low affinity red intensiometric genetically encoded Ca2+ indicator targeted to mitochondria, Addgene item #61245 (17) mito-mhYFP Mito-mhYFP, pcDNA3.1-derived vector encoding monomeric hyperfolder YFP fluorescent protein targeted to mitochondria, Addgene item #186526 (18) ..
Sequencing:Article Title: The Bordetella effector protein BteA induces host cell death by disruption of calcium homeostasis
Article Snippet: .. Plasmid Description Reference pBBRI MCS lacPOZ’ mob+, broad-host cloning vector, CmR (11, 12) pBBRI-PgroESmNG pBBRI vector with BbRB50 promotor GroES (PgroES) and coding sequence of the mNeonGreen protein (mNG) This study pBBRI-PfhaBmSc pBBRI vector with BpTohamaI filamentous hemagglutinin promotor (pfhaB) and coding sequence of the mScarlet protein (mSc) (13) export&KDELmScarlet ER-mScarletI, pEGFP-N1-derived vector encoding mScarlet fluorescent protein targeted to mitochondria, Addgene item # 137805 (14) 4xmtsmNeonGreen 4xmts-mNeonGreen, pEGFP-N1-derived vector encoding mNeonGreen fluorescent protein targeted to mitochondria, Addgene item # 98876 (14) GeCKO v2 sublibrary A Human CRISPR Knockout Pooled Library (GeCKO v2) in lentiGuide-Puro A, Catalog #1000000049, Addgene item #52959 (15) GeCKO v2 sublibrary B Human CRISPR Knockout Pooled Library (GeCKO v2) in lentiGuide-Puro B, Catalog #1000000049, Addgene item #52960 (15) pCMV-VSV-G Vector encoding envelope protein for producing lentiviral and MuLV retroviral particles, Addgene item #8454 (16) psPAX2 2nd generation lentiviral packaging plasmid, Addgene item #12260 Addgene, Trono Lab unpublished ER-LAR-GECO CMV-ER-LAR-GECO1, low affinity red intensiometric genetically encoded Ca2+ indicator targeted to ER, Addgene item #61244 (17) mito-LARGECO CMV-mito-LAR-GECO1.2, low affinity red intensiometric genetically encoded Ca2+ indicator targeted to mitochondria, Addgene item #61245 (17) mito-mhYFP Mito-mhYFP, pcDNA3.1-derived vector encoding monomeric hyperfolder YFP fluorescent protein targeted to mitochondria, Addgene item #186526 (18) ..
CRISPR:Article Title: The Bordetella effector protein BteA induces host cell death by disruption of calcium homeostasis
Article Snippet: .. Plasmid Description Reference pBBRI MCS lacPOZ’ mob+, broad-host cloning vector, CmR (11, 12) pBBRI-PgroESmNG pBBRI vector with BbRB50 promotor GroES (PgroES) and coding sequence of the mNeonGreen protein (mNG) This study pBBRI-PfhaBmSc pBBRI vector with BpTohamaI filamentous hemagglutinin promotor (pfhaB) and coding sequence of the mScarlet protein (mSc) (13) export&KDELmScarlet ER-mScarletI, pEGFP-N1-derived vector encoding mScarlet fluorescent protein targeted to mitochondria, Addgene item # 137805 (14) 4xmtsmNeonGreen 4xmts-mNeonGreen, pEGFP-N1-derived vector encoding mNeonGreen fluorescent protein targeted to mitochondria, Addgene item # 98876 (14) GeCKO v2 sublibrary A Human CRISPR Knockout Pooled Library (GeCKO v2) in lentiGuide-Puro A, Catalog #1000000049, Addgene item #52959 (15) GeCKO v2 sublibrary B Human CRISPR Knockout Pooled Library (GeCKO v2) in lentiGuide-Puro B, Catalog #1000000049, Addgene item #52960 (15) pCMV-VSV-G Vector encoding envelope protein for producing lentiviral and MuLV retroviral particles, Addgene item #8454 (16) psPAX2 2nd generation lentiviral packaging plasmid, Addgene item #12260 Addgene, Trono Lab unpublished ER-LAR-GECO CMV-ER-LAR-GECO1, low affinity red intensiometric genetically encoded Ca2+ indicator targeted to ER, Addgene item #61244 (17) mito-LARGECO CMV-mito-LAR-GECO1.2, low affinity red intensiometric genetically encoded Ca2+ indicator targeted to mitochondria, Addgene item #61245 (17) mito-mhYFP Mito-mhYFP, pcDNA3.1-derived vector encoding monomeric hyperfolder YFP fluorescent protein targeted to mitochondria, Addgene item #186526 (18) ..
Knock-Out:Article Title: The Bordetella effector protein BteA induces host cell death by disruption of calcium homeostasis
Article Snippet: .. Plasmid Description Reference pBBRI MCS lacPOZ’ mob+, broad-host cloning vector, CmR (11, 12) pBBRI-PgroESmNG pBBRI vector with BbRB50 promotor GroES (PgroES) and coding sequence of the mNeonGreen protein (mNG) This study pBBRI-PfhaBmSc pBBRI vector with BpTohamaI filamentous hemagglutinin promotor (pfhaB) and coding sequence of the mScarlet protein (mSc) (13) export&KDELmScarlet ER-mScarletI, pEGFP-N1-derived vector encoding mScarlet fluorescent protein targeted to mitochondria, Addgene item # 137805 (14) 4xmtsmNeonGreen 4xmts-mNeonGreen, pEGFP-N1-derived vector encoding mNeonGreen fluorescent protein targeted to mitochondria, Addgene item # 98876 (14) GeCKO v2 sublibrary A Human CRISPR Knockout Pooled Library (GeCKO v2) in lentiGuide-Puro A, Catalog #1000000049, Addgene item #52959 (15) GeCKO v2 sublibrary B Human CRISPR Knockout Pooled Library (GeCKO v2) in lentiGuide-Puro B, Catalog #1000000049, Addgene item #52960 (15) pCMV-VSV-G Vector encoding envelope protein for producing lentiviral and MuLV retroviral particles, Addgene item #8454 (16) psPAX2 2nd generation lentiviral packaging plasmid, Addgene item #12260 Addgene, Trono Lab unpublished ER-LAR-GECO CMV-ER-LAR-GECO1, low affinity red intensiometric genetically encoded Ca2+ indicator targeted to ER, Addgene item #61244 (17) mito-LARGECO CMV-mito-LAR-GECO1.2, low affinity red intensiometric genetically encoded Ca2+ indicator targeted to mitochondria, Addgene item #61245 (17) mito-mhYFP Mito-mhYFP, pcDNA3.1-derived vector encoding monomeric hyperfolder YFP fluorescent protein targeted to mitochondria, Addgene item #186526 (18) ..
Retroviral:Article Title: The Bordetella effector protein BteA induces host cell death by disruption of calcium homeostasis
Article Snippet: .. Plasmid Description Reference pBBRI MCS lacPOZ’ mob+, broad-host cloning vector, CmR (11, 12) pBBRI-PgroESmNG pBBRI vector with BbRB50 promotor GroES (PgroES) and coding sequence of the mNeonGreen protein (mNG) This study pBBRI-PfhaBmSc pBBRI vector with BpTohamaI filamentous hemagglutinin promotor (pfhaB) and coding sequence of the mScarlet protein (mSc) (13) export&KDELmScarlet ER-mScarletI, pEGFP-N1-derived vector encoding mScarlet fluorescent protein targeted to mitochondria, Addgene item # 137805 (14) 4xmtsmNeonGreen 4xmts-mNeonGreen, pEGFP-N1-derived vector encoding mNeonGreen fluorescent protein targeted to mitochondria, Addgene item # 98876 (14) GeCKO v2 sublibrary A Human CRISPR Knockout Pooled Library (GeCKO v2) in lentiGuide-Puro A, Catalog #1000000049, Addgene item #52959 (15) GeCKO v2 sublibrary B Human CRISPR Knockout Pooled Library (GeCKO v2) in lentiGuide-Puro B, Catalog #1000000049, Addgene item #52960 (15) pCMV-VSV-G Vector encoding envelope protein for producing lentiviral and MuLV retroviral particles, Addgene item #8454 (16) psPAX2 2nd generation lentiviral packaging plasmid, Addgene item #12260 Addgene, Trono Lab unpublished ER-LAR-GECO CMV-ER-LAR-GECO1, low affinity red intensiometric genetically encoded Ca2+ indicator targeted to ER, Addgene item #61244 (17) mito-LARGECO CMV-mito-LAR-GECO1.2, low affinity red intensiometric genetically encoded Ca2+ indicator targeted to mitochondria, Addgene item #61245 (17) mito-mhYFP Mito-mhYFP, pcDNA3.1-derived vector encoding monomeric hyperfolder YFP fluorescent protein targeted to mitochondria, Addgene item #186526 (18) ..
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